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KMID : 0545120100200030558
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 3 p.558 ~ p.562
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Differentiation of 3 Lactobacillus rhamnosus strains: E/N, Oxy and Pen by SDS-PAGE and two dimensional electrophoresis of surface-associated proteins
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Jarocki P.
Podlesny M.
Wasko A.
Siuda A.
Targonski Z.
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Abstract
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SDS-PAGE of extracted surface-associated proteins of Lactobacillus rhamnosus strains: E/N, Oxy and Pen was performed. The obtained protein patterns allowed differentiation of the examined strains, which was not accomplished by the commonly used RAPD genotypic method. The differentiation by SDS-PAGE method proved to be a useful tool for strain specific identification, which was further confirmed by 2DE analysis. Therefore, it can be used as an alternative or complementary method for both conventional and genotypic identification procedures, especially when closely related lactobacilli isolates are identified.
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KEYWORD
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Lactobacillus, polyacrylamide gel electrophoresis, random amplification of polymorphic DNA, surface-associated proteins
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